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KMID : 0368419970400020095
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Journal of Plant Biology
1997 Volume.40 No. 2 p.95 ~ p.101
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RESEARCH Suppression of Ca^2+-Influx after ACC-Uptake by Spermine in Vigna radiata
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Lee, Sang Choon
Choi, Min Seon/Lee, Sun Hi/Oh, Seung Eun
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Abstract
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Exogenous ACC-induced ethylene production in mung bean hypocotyl segments was severely inhibited by the treatment of spermine in a 6 hr incubation. In protoplasts acquired from the same tissues and pretreated with ACC, the concentration of cytosolic Ca^2+ was increased after the addition of exogenous Ca^2+. However, this increase of Ca^2+ concentration was strongly suppressed by spermine. In previous studies, an artifical increase of Ca^2+ influx by the treatment of a Ca^2+ -ionophore stimulated ACC-induced ethylene production. The inhibitory effect of spermine on ACC-induced ethylene production was more prominent than that of putrescine which has fewer NH^3+ groups than spermine. In addition, spermine more prominently suppressed ACC-induced ethylene production in protoplasts in which fewer Ca^2+ ions were released from Ca^2+ -storage organelles. Also, the amount of transcript of ACC-oxidase which converts ACC to ethylene was decreased by the treatment of spermine. However, this reduction resulted only through the suppression of ethylene production in a 2 hr incubation of mung bean segments. On the basis of these results, we suggest that there is a coupling of ACC-uptake to the increase of cytosolic Ca^2+ concentration. In addition, the reduction of exogenous ACC-induced ethylene production by spermine could have resulted, at least partially, from reducing the Ca^2+ influx which stimulates ACC-oxidase activity.
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